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SpeciesReactivity
mouse

Form
Stds=0-1000 ng/ml; Sample=100 ul; Sensitivity=25 ng/ml; 75 min assay; Quantitative; Ag-based Ab Assay; 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Purified Sm Coated plate (8 wells x 12 strips) 5406 2. Mouse anti-Sm IgG std A ( 0 ng/ml), 0.750ml 5407 3. Mouse anti-Sm IgG std B (62.5 ng/ml), 0.750ml. 5408 4. Mouse anti-Sm IgG std C (125 ng/ml), 0.750 ml. 5409 5. Mouse anti-Sm IgG std D (250 ng/ml), 0.750 ml. 5410 6. Mouse anti-Sm IgG std E (500 ng/ml), 0.750 ml. 5411 7. Mouse anti-Sm IgG std F (1000 ng/ml), 0.75 ml. 5412 8. Goat Anti-mouse IgG (H+L)-HRP conjugate (100X) 0.120 ml 5413 9. Sample Diluent, (10X), 10ml SD-10 10. Wash Solution (100X), 10 ml WB-100 11. TMB Substrate solution, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Complete Instruction Manual. M-5405

SpCrossreactivity
This kit measure anti-Sm Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.