The PA ELISA is to be used for the quantitative determination of PA. The assay will recognize both natural and recombinant PA. The kit has been configured for research use only and is not to be used in diagnostic procedures.

PRINCIPLE OF THE METHOD

The PA kit is a solid phase phase sandwich enzyme linked immuno sorbent assay（ELISA）. Samples , including standards of known PA concentrations and unknowns are pipetted into these wells. During the first incubation, the PAantigen and a biotinylated monoclonal antibody specific for PAare simultaneously incubated. After washing, the enzyme（streptavidin-peroxydase）is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PApresent in the samples.

REAGENTS PROVIDED AND RECONSTTTUTION

REAGENTS（Store at 2-8℃）	1×96 WELLS	0.5×96 WELLS	RECONSTTTUTION
96/48-wells microtiter plates	1	0.5	Ready-to-use
Plastiv cover	2	1	Ready-to-use
Standard: 40ng/ml	1Vials （0.6ml）	0.5Vials （0.3ml）	See reagents preparation on page 3
Blank control	1Vials （1.0ml）	1Vials （0.5ml）	Ready-to-use
Standard Diluent	1Vials （5ml）	1Vials （2.5ml）	Ready-to-use
Biotinylated anti-PA	1Vials （6ml）	1Vials （3.0ml）	Ready-to-use
Streptavidin-HRP	1Vials （10ml）	1Vials （5.0ml）	Ready-to-use
Washing Buffer	1Vials （60ml）	1Vials （30ml）	20× concentrate
Substrate A	1Vials （6.0ml）	1Vials （3.0ml）	Ready-to-use
Substrate B	1Vials 6.0ml）	1Vials （3.0ml）	Ready-to-use
Stopping Solution	1Vials （6.0ml）	1Vials （3.0ml）	Ready-to-use

MATERIAL REQUIRED BUT NOT PROVIDED

l Distilled water

l Pipettes:10ul、50ul、100ul、200ul、1000ul。

l Vortex mixer and magnetic stirrer.

INTENDED USE

PRINCIPLE OF THE METHOD

REAGENTS PROVIDED AND RECONSTTTUTION

REAGENTS（Store at 2-8℃）	1×96 WELLS	0.5×96 WELLS	RECONSTTTUTION
96/48-wells microtiter plates	1	0.5	Ready-to-use
Plastiv cover	2	1	Ready-to-use
Standard: 40ng/ml	1Vials （0.6ml）	0.5Vials （0.3ml）	See reagents preparation on page 3
Blank control	1Vials （1.0ml）	1Vials （0.5ml）	Ready-to-use
Standard Diluent	1Vials （5ml）	1Vials （2.5ml）	Ready-to-use
Biotinylated anti-PA	1Vials （6ml）	1Vials （3.0ml）	Ready-to-use
Streptavidin-HRP	1Vials （10ml）	1Vials （5.0ml）	Ready-to-use
Washing Buffer	1Vials （60ml）	1Vials （30ml）	20× concentrate
Substrate A	1Vials （6.0ml）	1Vials （3.0ml）	Ready-to-use
Substrate B	1Vials 6.0ml）	1Vials （3.0ml）	Ready-to-use
Stopping Solution	1Vials （6.0ml）	1Vials （3.0ml）	Ready-to-use

MATERIAL REQUIRED BUT NOT PROVIDED

l Distilled water

l Pipettes:10ul、50ul、100ul、200ul、1000ul。

l Vortex mixer and magnetic stirrer.

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Plant Phosphatidic acid ELISA kit

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