用于記憶CD8+T細(xì)胞的自助程序：CD40-CD40L信號(hào)的正反饋?zhàn)鳛槎螖U(kuò)展的關(guān)鍵決定因素

作者：Ja Shugart，S Bambina，AF Alice，R Montler，KS Bahjat

動(dòng)物

應(yīng)用信息

描述：

記憶性CD8+T細(xì)胞快速增殖和獲得溶細(xì)胞活性是保護(hù)細(xì)胞內(nèi)病原體免疫的關(guān)鍵。控制這一召回反應(yīng)的信號(hào)仍不清楚。提示當(dāng)全身炎癥受*，記憶CD8+T細(xì)胞自身產(chǎn)生CD40L是二次擴(kuò)張的重要催化劑。二次免疫同時(shí)伴有高水平的全身炎癥，導(dǎo)致CD8+T細(xì)胞繼發(fā)增殖，獨(dú)立于CD4+T細(xì)胞和CD40-CD40L信號(hào)。相反，當(dāng)炎癥反應(yīng)受*，記憶中CD8+T細(xì)胞的二次擴(kuò)張需要產(chǎn)生CD40L的細(xì)胞，而記憶的CD8+T細(xì)胞可以提供這一信號(hào)。這些結(jié)果表明，免疫接種方案對(duì)CD40L表達(dá)的CD8+T細(xì)胞的依賴性不同，提示CD8+T細(xì)胞的CD40L表達(dá)是一種在炎癥受*促進(jìn)記憶性CD8+T細(xì)胞二次擴(kuò)增的有效機(jī)制。

目標(biāo)：

小鼠靜脈注射單核細(xì)胞增多癥。取脾臟和肝臟在Omni Prep多樣本勻漿器上進(jìn)行勻漿.均質(zhì)被鍍以進(jìn)行菌落計(jì)數(shù)。

A Self-Help Program for Memory CD8+ T Cells: Positive Feedback via CD40-CD40L Signaling as a Critical Determinant of Secondary Expansion

Authors: JA Shugart, S Bambina, AF Alice, R Montler, KS Bahjat

Animal

Application Info

Description:

The ability of memory CD8+ T cells to rapidly proliferate and acquire cytolytic activity is critical for protective immunity against intracellular pathogens. The signals that control this recall response remain unclear. We show that CD40L production by memory CD8+ T cells themselves is an essential catalyst for secondary expansion when systemic inflammation is limited. Secondary immunization accompanied by high levels of systemic inflammation results in CD8+ T cell secondary expansion independent of CD4+ T cells and CD40-CD40L signaling. Conversely, when the inflammatory response is limited, memory CD8+ T cell secondary expansion requires CD40L-producing cells, and memory CD8+ T cells can provide this signal. These results demonstrate that vaccination regimens differ in their dependence on CD40L-expressing CD8+ T cells for secondary expansion, and propose that CD40L-expression by CD8+ T cells is a fail-safe mechanism that can promote memory CD8+ T cell secondary expansion when inflammation is limited.

Goal:

Mice were challenged with L. monocytogenes intravenously. Spleens and livers were harvested and homogenized on an Omni Prep multi-sample homogenizer. Homogenates were plated for colony enumeration.