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Normal Human Kidney Mesangial Cells

人腎小球系膜細胞

Normal Human Kidney Mesangial cells (NhKM) are found within the glomerulus and are derived from the cortex of the kidney during the isolation process. These cells “fill the gap" between adjacent capillary loops surrounded by the glomerular basement membrane (GBM) and the podocytes. The primary function of NhKM is to remove trapped residues and aggregated protein from the basement membrane.  In addition, they aid filtration by constituting part of the glomerular capillary tuft structure that filters fluids to produce urine.

The NhKM cells are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then NhKM cells are isolated by PDGF-R beta positive selection as a pure population, then cryopreserved.

Purity is analyzed using immunofluorescence microscopy for PDGFRβ, vimentin, and α smooth muscle actin. Cells are terminally differentiated and can be expanded for at least 15 population doublings. Mesangial cells have an intermediate phenotype, sharing features of fibroblasts and smooth muscle cells.

When grown in one of the common media formulations for eukaryotic cells (e.g. MEM, DMEM, Waymouth, RPMI 1640), these cells retain their in vivo phenotype as stellate, arborized elements in monolayer culture. Homogeneous cultures of Mesangial cells resemble smooth muscle cells or fibroblasts. On average, 15–20 passages in culture can been obtained from NhKM cells.

The NhKM cells are terminally differentiated.

THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.

Normal Human Kidney Mesangial Cells

人腎小球系膜細胞

PBMC分離試劑盒

人外周血單個核細胞分離試劑盒PBMC分離液

人PBMC分離試劑盒 人PBMC分離液

猴外周血單個核細胞分離試劑盒PBMC分離液

猴PBMC分離試劑盒 猴PBMC分離液

比格犬外周血單個核細胞分離試劑盒PBMC分離液

比格犬PBMC分離試劑盒 比格犬PBMC分離液

狗外周血單個核細胞分離試劑盒PBMC分離液

狗PBMC分離試劑盒 狗PBMC分離液

大鼠外周血單個核細胞分離試劑盒PBMC分離液

大鼠PBMC分離試劑盒 大鼠PBMC分離液

小鼠外周血單個核細胞分離試劑盒PBMC分離液

小鼠PBMC分離試劑盒 小鼠PBMC分離液

兔外周血單個核細胞分離試劑盒PBMC分離液

兔PBMC分離試劑盒 兔PBMC分離液

豬外周血單個核細胞分離試劑盒PBMC分離液

豬PBMC分離試劑盒 豬PBMC分離液

豚鼠外周血單個核細胞分離試劑盒PBMC分離液

豚鼠PBMC分離試劑盒 豚鼠PBMC分離液

骨髓單個核細胞分離試劑盒Bone Marrow Mononuclear Cells Isolation Kit

脾單個核細胞分離試劑盒 Spleen Mononuclear Cells Isolation Kit

紅細胞分離試劑盒

人紅細胞分離試劑盒 紅細胞分離液

猴紅細胞分離試劑盒 紅細胞分離液

比格犬紅細胞分離試劑盒 紅細胞分離液

大鼠紅細胞分離試劑盒 紅細胞分離液

小鼠紅細胞分離試劑盒 紅細胞分離液

ADME相關(guān)產(chǎn)品

Ⅰ相代謝穩(wěn)定性試劑盒

Ⅱ相代謝穩(wěn)定性試劑盒

CYP450 酶代謝表型研究試劑盒（化學(xué)抑制法/7種抑制劑）

CYP450 酶代謝表型研究試劑盒（重組酶法/7種酶）

CYP450 酶代謝表型研究試劑盒（重組酶法/單酶）

酶抑制（IC50）研究試劑盒（7種特異性底物）

酶抑制（IC50）研究試劑盒（單個酶）

NADPH再生系統(tǒng)

UGT孵育系統(tǒng)

0.1M PBS

肝微粒體（人，猴，犬，大鼠，小鼠）

肝S9（人，猴，犬，大鼠，小鼠）

肝原代細胞（人，猴，犬，大鼠，小鼠）

CYP450（CYP1A2，CYP2A6，CYP2B6，CYP2C8，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）

UGT酶

探針底物，代謝產(chǎn)物，抑制劑（CYP1A2，CYP2A6，CYP2B6，CYP2C8，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）

ADME服務(wù)項目

肝微粒體代謝穩(wěn)定性（人，猴，犬，大鼠，小鼠）

肝細胞代謝穩(wěn)定性試驗（人，猴，犬，大鼠，小鼠）

代謝表型研究

代謝產(chǎn)物鑒定

代謝途徑鑒定

種屬比較研究

CYPP450抑制實驗（CYP1A2，CYP2A6，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）

CYPP450誘導(dǎo)實驗

血漿蛋白結(jié)合率測定

血漿穩(wěn)定性試驗

跨膜轉(zhuǎn)運試驗

藥物-藥物相互作用

毒理學(xué)研究

Innovative Reagents for Innovative Research

匯智和源致力于為創(chuàng)新藥企業(yè)及生命科學(xué)研究機構(gòu)提供高品質(zhì)的生物試劑。